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Human Corticosteroid Binding Globulin (CBG) ELISA Kit

SKU:
HUDL00445
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08185
Sensitivity:
1.42ng/mL
Range:
3.125-200ng/mL
ELISA Type:
Sandwich
Synonyms:
SERPINA6,Transcortin,Serpin A6,Serpin Peptidase Inhibitor,Clade A,Member 6(Alpha-1 Antiproteinase,Antitrypsin)
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Corticosteroid Binding Globulin (CBG) ELISA Kit

The Human Corticosteroid Binding Globulin (CBG) ELISA Kit is a powerful tool for accurate detection of corticosteroid binding globulin levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.Corticosteroid binding globulin is an important carrier protein that binds cortisol and helps regulate the availability of cortisol in the body. Dysregulation of CBG levels has been linked to numerous health conditions, including diabetes, obesity, and stress-related disorders.

Therefore, measuring CBG levels is crucial for understanding these conditions and developing effective treatments.With its advanced technology and user-friendly design, the Human CBG ELISA Kit is the perfect solution for researchers seeking to study the role of corticosteroid binding globulin in health and disease. Order yours today and unlock the potential of your research.

Product Name:Human Corticosteroid Binding Globulin (CBG) ELISA Kit
Product Code:HUDL00445
Size:96 Assays
Target:CBG
Synonyms:SERPINA6, Transcortin, Serpin A6, Serpin Peptidase Inhibitor,Clade A,Member 6(Alpha-1 Antiproteinase, Antitrypsin)
Detection Method:Sandwich
Range:3.125-200ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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