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Human Cornulin (CRNN) ELISA Kit

SKU:
HUDL00686
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UBG3
Sensitivity:
0.123ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SEP53,C1orf10,DRC1,PDRC1,53 kDa putative calcium-binding protein,Squamous epithelial heat shock protein 53,Tumor-related protein,58 kDa heat shock protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Cornulin (CRNN) ELISA Kit

The Human Cornulin (CRNN) ELISA Kit is a reliable tool for detecting cornulin levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reproducible results for various research applications.Cornulin is a protein associated with epithelial differentiation and has been linked to skin disorders, oral cancer, and other epithelial-related diseases.

By measuring cornulin levels, researchers can gain valuable insights into disease progression and potential therapeutic targets.Overall, the Human Cornulin (CRNN) ELISA Kit is a valuable resource for studying the role of cornulin in various health conditions and developing targeted therapies for patients in need.

Product Name:Human Cornulin (CRNN) ELISA Kit
Product Code:HUDL00686
Size:96 Assays
Target:CRNN
Synonyms:SEP53, C1orf10, DRC1, PDRC1, 53 kDa putative calcium-binding protein, Squamous epithelial heat shock protein 53, Tumor-related protein, 58 kDa heat shock protein
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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