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Human Copeptin (CPP) ELISA Kit

SKU:
HUDL00657
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01185
Sensitivity:
5.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
C-Terminal Provasopressin,Vasopressin-Neurophysin 2-Copeptin
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Copeptin (CPP) ELISA Kit

The Human Copeptin (CPP) ELISA Kit is specifically developed for the precise measurement of copeptin levels in human samples such as serum, plasma, and cell culture supernatants. This innovative kit boasts exceptional sensitivity and selectivity, ensuring dependable and consistent results for various research purposes.Copeptin is a key biomarker that serves as a surrogate marker for the hormone vasopressin in the body.

It plays a vital role in regulating water balance and stress response, making it a valuable tool for studying conditions related to fluid balance, cardiovascular health, and neuroendocrine disorders. By accurately quantifying copeptin levels, researchers can gain deeper insights into these complex physiological processes and potentially uncover new therapeutic strategies.

Product Name:Human Copeptin (CPP) ELISA Kit
Product Code:HUDL00657
Size:96 Assays
Target:CPP
Synonyms:C-Terminal Provasopressin, Vasopressin-Neurophysin 2-Copeptin
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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