Human COMT Recombinant Protein (RPPB1502)
- SKU:
- RPPB1502
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P21964
- Research Area:
- Enzymes
Description
| Product Name: | Human COMT Recombinant Protein |
| Product Code: | RPPB1502 |
| Size: | 10µg |
| Species: | Human |
| Target: | COMT |
| Synonyms: | COMT, EC 2.1.1.6, Catechol O-methyltransferase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | COMT protein in 20mM Tris-HCl buffer, pH-8, 1mM MgCl2 and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P |
COMT catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to catechol substrates such as the neurotransmitters. This O-methylation results in one of the main degradative pathways of the catecholamine transmitters. COMT COMT is located in the postsynaptic neuron and is involved in the metabolism of catechol estrogen drugs used in the treatment of hypertension, asthma, Parkinson disease and the inactivation of catecholamine neurotransmitters though enzymatic degradation. COMT appears in tissues in 2 forms, a soluble form and a membrane-bound form which differ in their N-termini. COMT inhibitors increase its availability and are used in the treatment of patients with Parkinson's disease.
COMT Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 221 amino acids (51-271 a.a.) & having a molecular mass of 24.4 kDa. The COMT is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | COMT: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. Belongs to the mammalian catechol-O-methyltransferase family. 2 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Methyltransferase; EC 2.1.1.6; Amino Acid Metabolism - tyrosine Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: postsynaptic membrane; mitochondrion; membrane; axon; plasma membrane; dendritic spine; integral to membrane; cytosol Molecular Function:protein binding; magnesium ion binding; O-methyltransferase activity; catechol O-methyltransferase activity Biological Process: response to drug; methylation; estrogen metabolic process; cellular response to phosphate starvation; neurotransmitter catabolic process; dopamine catabolic process; negative regulation of smooth muscle cell proliferation; short-term memory; response to pain; response to lipopolysaccharide; female pregnancy; learning; reproductive process in a multicellular organism; response to organic cyclic substance; positive regulation of homocysteine metabolic process; negative regulation of dopamine metabolic process; synaptic transmission; xenobiotic metabolic process; regulation of sensory perception of pain; developmental process; neurotransmitter biosynthetic process Disease: Schizophrenia; Panic Disorder 1 |
| NCBI Summary: | Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008] |
| UniProt Code: | P21964 |
| NCBI GenInfo Identifier: | 116907 |
| NCBI Gene ID: | 1312 |
| NCBI Accession: | P21964.2 |
| UniProt Secondary Accession: | P21964,Q6IB07, Q6ICE6, Q9BWC7, A8MPV9, |
| UniProt Related Accession: | P21964 |
| Molecular Weight: | 271 |
| NCBI Full Name: | Catechol O-methyltransferase |
| NCBI Synonym Full Names: | catechol-O-methyltransferase |
| NCBI Official Symbol: | COMT�� |
| NCBI Official Synonym Symbols: | HEL-S-98n�� |
| NCBI Protein Information: | catechol O-methyltransferase; epididymis secretory sperm binding protein Li 98n |
| UniProt Protein Name: | Catechol O-methyltransferase |
| Protein Family: | Catechol O-methyltransferase |
| UniProt Gene Name: | COMT�� |
| UniProt Entry Name: | COMT_HUMAN |
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