The Human COL3 (Collagen Type III) ELISA Kit is designed for the quantitative determination of Collagen Type III levels in various human samples. Collagen Type III is a crucial structural protein that plays a significant role in the extracellular matrix, providing structural integrity and support to tissues. It is particularly abundant in tissues undergoing rapid growth or repair, contributing to the overall tissue architecture and function. Accurate measurement of Collagen Type III is essential as alterations in its levels can be indicative of pathological conditions, tissue remodeling, wound healing processes, and fibrosis-related disorders.
This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With stringent quality controls and ease of use, this kit delivers robust performance, making it well-suited for a wide range of research and clinical applications. Trust in Assay Genie's COL3 ELISA Kit for reliable and accurate quantification of this important biomarker in your studies.
Product Name:
Human COL3 (Collagen Type Ⅲ) ELISA Kit
SKU:
AEES00085
Target:
Human COL3 (Collagen Type Ⅲ)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL3. You can calculate the concentration of Human COL3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-94
85-100
100-108
Average (%)
91
92
104
1:4
Range (%)
95-106
87-99
100-109
Average (%)
99
94
105
1:8
Range (%)
96-108
92-107
98-106
Average (%)
102
99
101
1:16
Range (%)
98-104
98-106
99-107
Average (%)
101
101
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-100
92
EDTA plasma (n=5)
94-106
101
Cell culture media (n=5)
91-102
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.38
8.99
0.97
2.18
8.81
Standard deviation
0.05
0.12
0.41
0.05
0.1
0.36
C V (%)
5.43
5.04
4.56
5.15
4.59
4.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL3 in samples. No significant cross-reactivity or interference between Human COL3 and analogues was observed.
