Human COL3 alpha1 (Collagen Type III Alpha 1) CLIA Kit
The Human Col3 Alpha1 (Collagen Type III Alpha 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Col3 Alpha1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.Collagen Type III Alpha 1 is a key component of the extracellular matrix, playing a crucial role in maintaining tissue integrity and providing structural support.
Changes in Col3 Alpha1 levels have been associated with various pathological conditions, including cardiovascular diseases, fibrosis, and connective tissue disorders.By accurately measuring Col3 Alpha1 levels, researchers can gain valuable insights into the pathophysiology of these diseases and potentially identify new therapeutic targets. The Human Col3 Alpha1 CLIA Kit offers a valuable tool for studying the role of Collagen Type III Alpha 1 in health and disease.
Product Name:
Human COL3 alpha1 (Collagen Type III Alpha 1) CLIA Kit
SKU:
HUES00488
Target:
Human COL3 alpha1 (Collagen Type III Alpha 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL3α1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL3α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL3α1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL3α1. You can calculate the concentration of Human COL3α1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
93-108
87-103
Average (%)
109
100
94
1:4
Range (%)
101-118
86-97
95-106
Average (%)
109
92
100
1:8
Range (%)
94-108
85-97
90-105
Average (%)
101
91
96
1:16
Range (%)
94-105
83-95
93-106
Average (%)
99
90
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
97-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
426.1
846.34
2747.87
430.25
904.09
2729.01
Standard deviation
46.02
97.08
205.54
44.27
86.61
229.51
C V (%)
10.8
11.47
7.48
10.29
9.58
8.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL3α1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL3α1 in samples. No significant cross-reactivity or interference between Human COL3α1 and analogues was observed.
