The Human COL17 (Collagen Type XVII) ELISA Kit is a reliable and accurate tool for detecting levels of collagen type XVII in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Collagen type XVII is a key protein involved in maintaining the integrity of skin and other tissues, playing a crucial role in cell adhesion and migration. Dysregulation of collagen type XVII has been linked to various skin disorders and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With the Human COL17 ELISA Kit, researchers can confidently measure levels of collagen type XVII in human samples, advancing their understanding of tissue homeostasis and disease pathology. Trust in this kit for accurate and reliable results in your research endeavors.
Product Name:
Human COL17 (Collagen Type XVII) ELISA Kit
SKU:
HUES01923
Target:
Human COL17 (Collagen Type XVII)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL17. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL17, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL17. You can calculate the concentration of Human COL17 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
87-102
85-95
Average (%)
95
93
90
1:4
Range (%)
88-100
85-100
88-100
Average (%)
95
92
95
1:8
Range (%)
86-98
79-90
82-98
Average (%)
93
85
89
1:16
Range (%)
91-103
82-94
85-97
Average (%)
97
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
101
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.57
26.33
87.89
10.51
26.52
91.96
Standard deviation
0.65
1.43
3.7
0.73
1.47
3.87
C V (%)
6.79
5.43
4.21
6.95
5.54
4.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL17 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL17 in samples. No significant cross-reactivity or interference between Human COL17 and analogues was observed.
