Human COL1 alpha1 (Collagen Type I Alpha 1) ELISA Kit
The Human Collagen Type I Alpha 1 ELISA Kit is specially designed for the precise measurement of collagen type I alpha 1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, guaranteeing accurate and consistent results, which makes it perfect for a variety of research purposes.Collagen type I alpha 1 is a key component of collagen, a structural protein that plays a crucial role in maintaining the integrity and strength of tissues such as skin, bone, and tendons.
Changes in collagen type I alpha 1 levels have been linked to various health conditions, including skin disorders, osteoporosis, and connective tissue diseases, making it a valuable biomarker for studying these diseases and developing potential treatments. For researchers looking to explore the role of collagen type I alpha 1 in health and disease, the Human Collagen Type I Alpha 1 ELISA Kit provides a reliable and effective tool for accurate quantification and analysis.
Product Name:
Human COL1 alpha1 (Collagen Type I Alpha 1) ELISA Kit
SKU:
HUES01997
Target:
Human COL1 alpha1 (Collagen Type I Alpha 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL1α1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL1α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL1α1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL1α1. You can calculate the concentration of Human COL1α1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
89-102
88-103
Average (%)
103
96
94
1:4
Range (%)
88-102
81-95
83-97
Average (%)
96
88
88
1:8
Range (%)
89-100
80-93
88-100
Average (%)
95
86
95
1:16
Range (%)
92-104
79-91
88-101
Average (%)
97
85
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-97
92
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
3.05
8.08
1.0
2.83
8.21
Standard deviation
0.06
0.16
0.38
0.07
0.16
0.43
C V (%)
6
5.25
4.7
7.0
5.65
5.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL1α1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL1α1 in samples. No significant cross-reactivity or interference between Human COL1α1 and analogues was observed.
