Human Coagulation Factor XIII A1 Polypeptide (F13A1) ELISA Kit
The Human Coagulation Factor XIII A1 Polypeptide (F13A1) ELISA Kit is a reliable and accurate tool for measuring levels of the F13A1 protein in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and reproducible results for a wide range of research applications.Coagulation Factor XIII A1 is a key component in the blood clotting cascade, playing a crucial role in the formation and stabilization of blood clots. Dysregulation of F13A1 has been linked to various bleeding disorders and thrombotic conditions, making it a valuable biomarker for understanding these diseases and developing targeted treatments.
With the Human Coagulation Factor XIII A1 Polypeptide (F13A1) ELISA Kit, researchers can accurately measure F13A1 levels in biological samples, allowing for in-depth studies on the role of this protein in health and disease. This kit is a valuable tool for researchers working in the fields of hematology, coagulation disorders, and thrombosis.
Product Name:
Human Coagulation Factor XIII A1 Polypeptide (F13A1) ELISA Kit
Product Code:
HUDL00996
Size:
96 Assays
Target:
F13A1
Synonyms:
F13-A1, FXIIIA1, Protein-glutamine gamma-glutamyltransferase A chain, Transglutaminase A chain, Coagulation factor XIII A chain
Detection Method:
Sandwich
Range:
18.75-1200pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
