Human CNTF Recombinant Protein (RPPB0127)
- SKU:
- RPPB0127
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P26441
- Research Area:
- Neurotrophins
Description
| Product Name: | Human CNTF Recombinant Protein |
| Product Code: | RPPB0127 |
| Size: | 20µg |
| Species: | Human |
| Target: | CNTF |
| Synonyms: | HCNTF, CNTF, Ciliary Neurotrophic Factor. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a concentrated (1mg/ml) solution in water containing 5mM sodium Phosphate buffer pH=7.5 and 5mM sodium chloride. |
| Solubility: | It is recommended to reconstitute the lyophilized HCNTF in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Ciliary Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CNTF should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Phe-Thr-Glu |
| Biological Activity: | The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 500,000IU/mg. |
CNTF is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor.CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Ciliary Neurotrophic Factor Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 22706 Dalton. The CNTF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 11q12.2 Cellular Component: cytoplasm; extracellular region; extracellular space Molecular Function:ciliary neurotrophic factor receptor binding; growth factor activity; interleukin-6 receptor binding; protein binding Biological Process: growth; muscle morphogenesis; negative regulation of neuron apoptosis; negative regulation of photoreceptor cell differentiation; neuron development; positive regulation of cell proliferation; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of retinal cell programmed cell death; signal transduction |
| NCBI Summary: | The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010] |
| UniProt Code: | P26441 |
| NCBI GenInfo Identifier: | 116585 |
| NCBI Gene ID: | 1270 |
| NCBI Accession: | P26441.1 |
| UniProt Secondary Accession: | P26441,B2RAB2, |
| UniProt Related Accession: | P26441 |
| Molecular Weight: | |
| NCBI Full Name: | Ciliary neurotrophic factor |
| NCBI Synonym Full Names: | ciliary neurotrophic factor |
| NCBI Official Symbol: | CNTF�� |
| NCBI Official Synonym Symbols: | HCNTF�� |
| NCBI Protein Information: | ciliary neurotrophic factor |
| UniProt Protein Name: | Ciliary neurotrophic factor |
| Protein Family: | Ciliary neurotrophic factor |
| UniProt Gene Name: | CNTF�� |
| UniProt Entry Name: | CNTF_HUMAN |
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