The Human CNTF (Ciliary Neurotrophic Factor) CLIA Kit is a highly reliable and sensitive assay designed for the accurate measurement of CNTF levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides researchers with the tools needed to study the role of CNTF in various biological processes.CNTF is a key neurotrophic factor that plays a critical role in the survival and differentiation of neurons. It has been implicated in a variety of neurological disorders, including motor neuron disease and Alzheimer's disease.
By accurately measuring CNTF levels, researchers can gain valuable insight into the mechanisms underlying these conditions and potentially develop new therapeutic interventions.With its high sensitivity and specificity, the Human CNTF CLIA Kit offers reliable and reproducible results, making it an essential tool for any research focused on understanding the role of CNTF in human health and disease.
Product Name:
Human CNTF (Ciliary Neurotrophic Factor) CLIA Kit
SKU:
HUES00038
Target:
Human CNTF (Ciliary Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CNTF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CNTF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CNTF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CNTF. You can calculate the concentration of Human CNTF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
102-116
90-101
Average (%)
100
108
95
1:4
Range (%)
100-116
101-116
91-104
Average (%)
106
108
98
1:8
Range (%)
95-110
83-96
99-112
Average (%)
101
90
106
1:16
Range (%)
102-114
84-96
94-107
Average (%)
109
91
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-117
108
EDTA plasma (n=5)
91-104
96
Cell culture media (n=5)
98-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.87
114.2
464.28
48.01
115.57
441.42
Standard deviation
5.28
9.35
43.27
6.0
13.78
32.14
C V (%)
11.51
8.19
9.32
12.5
11.92
7.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CNTF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CNTF in samples. No significant cross-reactivity or interference between Human CNTF and analogues was observed.
