Human CNN1 (Calponin 1, Basic, Smooth Muscle) CLIA Kit
The Human CNN1 (Calponin 1) Basic Smooth Muscle CLIA Kit is a cutting-edge assay designed for the precise and reliable measurement of calponin 1 levels in human samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Calponin 1 is a key protein involved in the regulation of smooth muscle contraction, making it essential for maintaining vascular tone and organ function. Dysregulation of calponin 1 has been implicated in various diseases, including cardiovascular disorders and smooth muscle-related pathologies, highlighting its significance as a potential therapeutic target and biomarker.
By utilizing the Human CNN1 Basic Smooth Muscle CLIA Kit, researchers can gain valuable insights into the role of calponin 1 in health and disease, paving the way for the development of novel diagnostic tools and treatment strategies. Get your hands on this advanced assay kit today and elevate your research to new heights.
Product Name:
Human CNN1 (Calponin 1, Basic, Smooth Muscle) CLIA Kit
SKU:
HUES00246
Target:
Human CNN1 (Calponin 1, Basic, Smooth Muscle)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CNN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CNN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CNN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CNN1. You can calculate the concentration of Human CNN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
89-101
102-115
Average (%)
95
95
108
1:4
Range (%)
93-108
85-96
89-101
Average (%)
99
91
94
1:8
Range (%)
85-98
96-111
84-97
Average (%)
90
102
91
1:16
Range (%)
92-104
89-100
101-120
Average (%)
99
95
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
106
EDTA plasma (n=5)
100-113
106
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
255.84
652.22
2463.1
237.67
598.27
2421.34
Standard deviation
23.54
61.9
202.96
25.29
52.89
181.12
C V (%)
9.2
9.49
8.24
10.64
8.84
7.48
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CNN1 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human CNN1 in samples. No significant cross-reactivity or interference between Human CNN1 and analogues was observed.
