Human CMBL Recombinant Protein (RPPB1497)
- SKU:
- RPPB1497
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q96DG6
- Research Area:
- Enzymes
Description
| Product Name: | Human CMBL Recombinant Protein |
| Product Code: | RPPB1497 |
| Size: | 20µg |
| Species: | Human |
| Target: | CMBL |
| Synonyms: | Carboxymethylenebutenolidase homolog, CMBL, JS-1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The CMBL solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMANEAY PCPCDIGHRL EYGGLGREVQ VEHIKAYVTK SPVDAGKAVI VIQDIFGWQL PNTRYIADMI SGNGYTTIVP DFFVGQEPWD PSGDWSIFPE WLKTRNAQKI DREISAILKY LKQQCHAQKI GIVGFCWGGT AVHHLMMKYS EFRAGVSVYG IVKDSEDIYN LKNPTLFIFA ENDVVIPLKD VSLLTQKLKE HCKVEYQIKT FSGQTHGFVH RKREDCSPAD KPYIDEARRN LIEWLNKYM |
Carboxymethylenebutenolidase homolog (CMBL) is a cysteine hydrolase of the dienelactone hydrolase family which is highly expressed in the liver cytosol. CMBL is the human homolog of Pseudomonas dienelactone hydrolase, which is a protein that participates in the bacterial halocatechol degradation pathway. CMBL which preferentially cleaves cyclic esters activates medoxomil-ester prodrugs in which the medoxomil moiety is coupled with an oxygen atom. CMBL is inhibited by PCMB (p-chloromercuribenzoate) and is encoded by a gene which maps to human chromosome 5p15.2. CMBL can also activate beta-lactam antibiotics faropenem medoxomil and lenampicillin. CMBL is widely expressed, with the highest levels in the liver, followed by the kidney, small intestine and the colon.
CMBL Human Recombinant produced in E. coli is a single polypeptide chain containing 269 amino acids (1-245) and having a molecular mass of 30.6kDa.CMBL is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CMBL: Cysteine hydrolase. Can convert the prodrug olmesartan medoxomil into its pharmacologically active metabolite olmerstatan, an angiotensin receptor blocker, in liver and intestine. May also activate beta-lactam antibiotics faropenem medoxomil and lenampicillin. Belongs to the dienelactone hydrolase family.Protein type: Hydrolase; EC 3.1.-.-Chromosomal Location of Human Ortholog: 5p15.2Cellular Component: cytosolMolecular Function: hydrolase activityBiological Process: xenobiotic metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | CMBL (EC 3.1.1.45) is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom (Ishizuka et al., 2010 [PubMed 20177059]).[supplied by OMIM, Apr 2010] |
| UniProt Code: | Q96DG6 |
| NCBI GenInfo Identifier: | 20270371 |
| NCBI Gene ID: | 134147 |
| NCBI Accession: | NP_620164.1 |
| UniProt Secondary Accession: | Q96DG6,Q8TED6, D3DTC7 |
| UniProt Related Accession: | Q96DG6 |
| Molecular Weight: | 28 kDa |
| NCBI Full Name: | carboxymethylenebutenolidase homolog |
| NCBI Synonym Full Names: | carboxymethylenebutenolidase homolog (Pseudomonas) |
| NCBI Official Symbol: | CMBL�� |
| NCBI Official Synonym Symbols: | JS-1�� |
| NCBI Protein Information: | carboxymethylenebutenolidase homolog |
| UniProt Protein Name: | Carboxymethylenebutenolidase homolog |
| UniProt Synonym Protein Names: | |
| Protein Family: | Carboxymethylenebutenolidase |
| UniProt Gene Name: | CMBL�� |
| UniProt Entry Name: | CMBL_HUMAN |
