The Human CMA1 (Chymase 1, Mast Cell) ELISA Kit is a cutting-edge assay crafted for the precise quantitative assessment of CMA1 protein levels in human samples. CMA1, a pivotal enzyme predominantly expressed in mast cells, plays a fundamental role in mediating various biological processes such as inflammation, tissue remodeling, and immune responses. Dysregulation of CMA1 has been associated with inflammatory disorders, allergies, and other immune-related conditions, underscoring the importance of accurate measurement of CMA1 levels for unraveling mast cell functions and immune system modulation. With outstanding sensitivity and specificity, the Human CMA1 ELISA Kit empowers researchers to quantitatively analyze CMA1 expression levels, facilitating investigations into mast cell activation, inflammatory pathways, and immune regulatory mechanisms.
By providing reliable data on CMA1 protein concentrations, this ELISA kit serves as a valuable tool for researchers seeking to deepen their understanding of mast cell biology, inflammatory responses, and immune-related disorders. Manufactured under rigorous quality control standards, the Human CMA1 ELISA Kit ensures robust and reproducible performance in quantifying CMA1, offering researchers a reliable platform to advance their studies in immunology, allergy research, and inflammatory disease investigations. Researchers can leverage this user-friendly kit to explore mast cell functionality, decipher immune responses, and explore the potential therapeutic implications related to CMA1 modulation.
Product Name:
Human CMA1 (Chymase 1, Mast Cell) ELISA Kit
SKU:
AEES00673
Target:
Human CMA1 (Chymase 1, Mast Cell)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CMA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CMA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CMA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CMA1. You can calculate the concentration of Human CMA1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
86-101
100-116
Average (%)
97
92
106
1:4
Range (%)
93-108
86-101
97-114
Average (%)
99
93
105
1:8
Range (%)
86-101
88-103
98-111
Average (%)
93
94
105
1:16
Range (%)
87-99
83-97
102-119
Average (%)
94
90
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
87-101
92
Cell culture media (n=5)
93-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
276.09
648.72
2043.14
290.74
605.11
2165.0
Standard deviation
17.45
36.98
68.45
15.67
28.32
105.65
C V (%)
6.32
5.7
3.35
5.39
4.68
4.88
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CMA1 concentrations in .
Specificity:
This kit recognizes Human CMA1 in samples. No significant cross-reactivity or interference between Human CMA1 and analogues was observed.
