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Human Cluster Of Differentiation 8a (CD8a) ELISA Kit

SKU:
HUDL00498
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01732
Sensitivity:
1.3ng/mL
Range:
3.125-200ng/mL
ELISA Type:
Sandwich
Synonyms:
p32,CD8-A,Leu2,MAL,T-cell surface glycoprotein CD8 alpha chain,T-lymphocyte differentiation antigen T8/Leu-2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

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Human Cluster Of Differentiation 8a (CD8a) ELISA Kit

The Human Cluster of Differentiation 8A (CD8A) ELISA Kit is specifically designed for the accurate quantification of CD8A levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.CD8A, also known as Cluster of Differentiation 8A, is a cell surface glycoprotein found on cytotoxic T cells, and plays a crucial role in immune response by recognizing and binding to antigens presented by MHC class I molecules.

Measurement of CD8A levels can provide valuable insights into immune function, autoimmune diseases, and immunodeficiencies.With its advanced technology and user-friendly format, the Human CD8A ELISA Kit is a valuable tool for researchers studying immune responses, infectious diseases, and immunotherapies. Trust in the accuracy and reproducibility of this kit to drive your research forward.

Product Name:Human Cluster Of Differentiation 8a (CD8a) ELISA Kit
Product Code:HUDL00498
Size:96 Assays
Target:CD8a
Synonyms:p32, CD8-A, Leu2, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Detection Method:Sandwich
Range:3.12-200ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)84-10293
EDTA plasma (n=5)91-10095
Heparin plasma (n=5)92-9895
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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