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Human Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

SKU:
HUDL00492
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P29965
Sensitivity:
0.051ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD154,TRAP,HIGM1,IGM,IMD3,TBAM,T-BAM,TNFSF5,Gp39,TNF Superfamily Member 5,Hyper-IgM Syndrome,TNF-Related Activation Protein,T-Cell B-Cell Activating Molecule
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

The Human Cluster of Differentiation 40 Ligand (CD40L) ELISA Kit is a high-quality assay designed for the precise quantification of CD40L levels in human samples, including serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.CD40L, also known as CD154, is a critical protein involved in immune response regulation and inflammation. It plays a key role in various diseases, including autoimmune disorders, infectious diseases, and cardiovascular conditions.

Monitoring CD40L levels is essential for understanding disease pathology and identifying potential therapeutic targets.With its reliable performance and comprehensive information, the Human CD40L ELISA Kit is an invaluable tool for researchers studying immune system function, inflammatory processes, and disease development. Get accurate and reliable results with this advanced ELISA kit from Assay Genie.

Product Name:Human Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit
Product Code:HUDL00492
Size:96 Assays
Target:CD40L
Synonyms:CD154, TRAP, HIGM1, IGM, IMD3, TBAM, T-BAM, TNFSF5, Gp39, TNF Superfamily Member 5, Hyper-IgM Syndrome, TNF-Related Activation Protein, T-Cell B-Cell Activating Molecule
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-10296
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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