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Human Cluster Of Differentiation 4 (CD4) ELISA Kit

SKU:
HUDL00493
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01730
Sensitivity:
0.127ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD4mut,p55,T-cell surface glycoprotein CD4,T-cell surface antigen T4/Leu-3
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Cluster Of Differentiation 4 (CD4) ELISA Kit

The Human Cluster of Differentiation 4 (CD4) ELISA Kit is specially designed for the precise measurement of CD4 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it suitable for various research purposes.CD4 is a critical protein found on the surface of T cells, which are key players in the immune system.

Monitoring CD4 levels is essential for understanding immune function and immune response in conditions such as HIV/AIDS, autoimmune disorders, and infectious diseases. This ELISA kit provides a reliable tool for researchers to study the role of CD4 in these conditions and explore potential therapeutic interventions.

Product Name:Human Cluster Of Differentiation 4 (CD4) ELISA Kit
Product Code:HUDL00493
Size:96 Assays
Target:CD4
Synonyms:CD4mut, p55, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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