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Human Cluster Of Differentiation 276 (CD276) ELISA Kit

SKU:
HUDL00487
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q5ZPR3
Sensitivity:
0.115ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
B7-H3,B7H3,CD276 Molecule,B7 homolog 3,Costimulatory molecule
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Cluster Of Differentiation 276 (CD276) ELISA Kit

The Human CD276 (Cluster of Differentiation 276) ELISA Kit is a reliable tool for detecting CD276 levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reproducible results for a variety of research applications.CD276 is a cell surface protein that plays a key role in regulating immune responses and promoting tumor growth. Its expression has been linked to various cancers, making it a valuable biomarker for cancer research and potential therapeutic development.

This ELISA kit is easy to use and provides rapid results, making it a valuable tool for studying CD276's role in cancer progression and immune regulation. Researchers and clinicians alike can benefit from the valuable insights gained from utilizing this kit in their studies.

Product Name:Human Cluster Of Differentiation 276 (CD276) ELISA Kit
Product Code:HUDL00487
Size:96 Assays
Target:CD276
Synonyms:Azoreductase,DIA4,DTD,DT-diaphorase,Menadione reductase,NAD(P)H dehydrogenase [quinone] 1,NAD(P)H:quinone oxidoreductase 1,NMOR1,Phylloquinone reductase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-10095
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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