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Human Cluster Of Differentiation 200 (CD200) ELISA Kit

SKU:
HUDL00481
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P41217
Sensitivity:
5.1pg/mL
Range:
12.5-800pg/mL
ELISA Type:
Sandwich
Synonyms:
MOX1,MOX2,MRC,OX-2,My033,Antigen Identified By Monoclonal Antibody MRC OX-2
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Cluster Of Differentiation 200 (CD200) ELISA Kit

The Human Cluster of Differentiation 200 (CD200) ELISA Kit from Assay Genie is a specially designed assay for the accurate measurement of CD200 levels in human samples. This kit offers high sensitivity and specificity, providing dependable and consistent results suitable for various research applications. CD200 is a cell surface glycoprotein that plays a pivotal role in immunoregulation and immune tolerance. Dysregulation of CD200 has been linked to autoimmune diseases, chronic inflammation, and cancer progression, making it a valuable biomarker for studying these conditions and exploring therapeutic strategies.

With the Human CD200 ELISA Kit, researchers can easily quantify CD200 levels in serum, plasma, and cell culture supernatants, facilitating detailed investigations into immune responses and disease mechanisms. Trust Assay Genie for reliable and robust ELISA kits to support your cutting-edge research endeavors.

Product Name:Human Cluster Of Differentiation 200 (CD200) ELISA Kit
Product Code:HUDL00481
Size:96 Assays
Target:CD200
Synonyms:MOX1, MOX2, MRC, OX-2, My033, Antigen Identified By Monoclonal Antibody MRC OX-2
Detection Method:Sandwich
Range:12.5-800pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-10296
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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