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Human Cluster Of Differentiation 160 (CD160) ELISA Kit

SKU:
HUDL00478
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O95971
Sensitivity:
30pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
BY55,NK1,NK28,CD160 Antigen,Natural killer cell receptor BY55
Reactivity:
Human
Sample Type:
Serum, Plasma, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Cluster Of Differentiation 160 (CD160) ELISA Kit

The Human CD160 (Cluster of Differentiation 160) ELISA Kit is specifically designed for the precise quantification of CD160 levels in human serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.CD160 is a cell surface glycoprotein that is an important regulator of immune responses and has been implicated in various inflammatory and autoimmune diseases.

By measuring CD160 levels, researchers can gain valuable insights into the immune system's function and potential therapeutic targets for immune-related disorders.Overall, the Human CD160 ELISA Kit is an indispensable tool for researchers studying immune function, inflammation, and autoimmune diseases, providing reliable data for advancing our understanding of CD160's role in health and disease.

Product Name:Human Cluster Of Differentiation 160 (CD160) ELISA Kit
Product Code:HUDL00478
Size:96 Assays
Target:CD160
Synonyms:BY55, NK1, NK28, CD160 Antigen, Natural killer cell receptor BY55
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9994
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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