The Human CLDN4 (Claudin-4) ELISA Kit is a powerful tool for measuring levels of claudin-4 in human biological samples such as serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit provides reliable and reproducible results for a variety of research applications.Claudin-4 is a key protein involved in cell adhesion and the formation of tight junctions, playing a crucial role in maintaining the integrity of epithelial cell barriers.
Dysregulation of claudin-4 has been associated with various diseases, including cancer, inflammatory bowel disease, and viral infections. Therefore, this ELISA kit serves as a valuable tool for studying the role of claudin-4 in these conditions and may aid in the development of novel treatment strategies.
Product Name:
Human CLDN4 (Claudin 4) ELISA Kit
SKU:
HUES01941
Target:
Human CLDN4 (Claudin 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CLDN4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLDN4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLDN4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CLDN4. You can calculate the concentration of Human CLDN4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
93-109
90-103
Average (%)
99
100
96
1:4
Range (%)
90-103
83-94
84-99
Average (%)
95
88
91
1:8
Range (%)
90-101
79-92
88-98
Average (%)
96
85
93
1:16
Range (%)
87-102
85-96
89-99
Average (%)
93
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
87-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.28
3.76
0.55
1.3
4.07
Standard deviation
0.03
0.06
0.13
0.03
0.07
0.15
C V (%)
5.88
4.69
3.46
5.45
5.38
3.69
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CLDN4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLDN4 in samples. No significant cross-reactivity or interference between Human CLDN4 and analogues was observed.
