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Human CLDN1 / Claudin-1 ELISA Kit

SKU:
HUFI02327
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O95832
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
CLDN1, Senescence-associated epithelial membrane protein, CLD1, claudin 1, ILVASC, CLD1, CLDN1, SEMP1, claudin 1, claudin-1, CLD1, ILVASC, Senescence-associated epithelial membrane protein, SEMP1, senescence-associated epithelial membrane protein 1
Reactivity:
Human
Research Area:
Immunology
€599
Frequently bought together:

Description

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Human CLDN1/Claudin-1 ELISA Kit

The Human CLDN1 (Claudin-1) ELISA Kit is expertly designed for precise and accurate measurement of CLDN1 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit guarantees dependable and reproducible results, making it an invaluable tool for a wide array of research applications.CLDN1, also known as Claudin-1, is a vital protein involved in the regulation of tight junctions between cells, playing a crucial role in maintaining epithelial barrier functions and cell polarity.

Dysregulation of CLDN1 has been implicated in various diseases, including cancer, inflammatory bowel disease, and hepatitis, underscoring its importance as a biomarker for disease progression and therapeutic interventions.Experience the precision and reliability of the Human CLDN1 ELISA Kit in your research endeavors, paving the way for new insights and discoveries in the field of molecular biology and pathology.

Product Name:Human CLDN1 / Claudin-1 ELISA Kit
Product Code:HUFI02327
Size:96 Assays
Alias:CLDN1, Senescence-associated epithelial membrane protein, CLD1, claudin 1, ILVASC, CLD1, CLDN1, SEMP1, claudin 1, claudin-1, CLD1, ILVASC, Senescence-associated epithelial membrane protein, SEMP1, senescence-associated epithelial membrane protein 1
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human CLDN1 concentrations in serum plasma and other biological fluids.
Sensitivity:0.094ng/ml
Range:0.156-10ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human CLDN1 and the recovery rates were calculated by comparing the measured value to the expected amount of Human CLDN1 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)87-10594
EDTA plasma(n=5)87-10597
UFH plasma(n=5)92-10297
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human CLDN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)87-95%85-104%87-103%
EDTA plasma(n=5)83-98%84-98%82-98%
UFH plasma(n=5)80-97%81-100%85-96%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotO95832
UniProt Protein Function:Claudin-1: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Acts as a co- receptor for HCV entry into hepatic cells. Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and INADL. May interact with HCV E1 and E2 proteins. Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. Belongs to the claudin family.
UniProt Protein Details:Protein type:Membrane protein, integral; Cytoskeletal; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: integral to membrane; integral to plasma membrane; tight junction

Molecular Function:identical protein binding; protein binding

Biological Process: calcium-independent cell-cell adhesion; intercellular junction assembly and maintenance; protein heterooligomerization; protein homooligomerization

Disease: Ichthyosis, Leukocyte Vacuoles, Alopecia, And Sclerosing Cholangitis

NCBI Summary:Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]
UniProt Code:O95832
NCBI GenInfo Identifier:6685283
NCBI Gene ID:9076
NCBI Accession:O95832.1
UniProt Related Accession:O95832
Molecular Weight:22,744 Da
NCBI Full Name:Claudin-1
NCBI Synonym Full Names:claudin 1
NCBI Official Symbol:CLDN1  
NCBI Official Synonym Symbols:CLD1; SEMP1; ILVASC  
NCBI Protein Information:claudin-1
UniProt Protein Name:Claudin-1
UniProt Synonym Protein Names:Senescence-associated epithelial membrane protein
Protein Family:Claudin
UniProt Gene Name:CLDN1  
UniProt Entry Name:CLD1_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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