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Human CKMT1 Recombinant Protein (RPPB2481)

SKU:
RPPB2481
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P06732
Research Area:
Enzymes
€309
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Description

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Product Name:Human CKMT1 Recombinant Protein
Product Code:RPPB2481
Size:50µg
Species:Human
Target:CKMT1
Synonyms:Creatine kinase M-type, EC 2.7.3.2, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITI.
Source:Pichia Pastoris
Physical Appearance:Sterile Filtered colourless liquid formulation.
Formulation:CKMT1 contains 0.01M Tris-HCl, 0.075M NaCl, 10mM beta-mercaptoethanol, 50% glycerol, 0.1% sodium azide, pH 7.2.
Stability:CKMT1 although stable at 15°C for 7 days, should be stored below -18°C. Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined bya) Analysis by RP-HPLCb) Analysis by SDS-PAGE
Biological Activity:The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 �mole creatine phosphate was 537IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,863ng/ml.

Creatine Kinase MM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

CKMT1 Human Recombinant without C-terminal Lysine on both chains produced in Pichia Pastoris is a glycosylated 47kDa polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MM Isoenzyme in ELISA. The CKMT1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - arginine and proline; Kinase, other; EC 2.7.3.2

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol

Molecular Function:creatine kinase activity; ATP binding

Biological Process: phosphocreatine biosynthetic process; phosphorylation; creatine metabolic process

NCBI Summary:The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]
UniProt Code:P06732
NCBI GenInfo Identifier:125305
NCBI Gene ID:1158
NCBI Accession:P06732.2
UniProt Secondary Accession:P06732,Q96QL9,
UniProt Related Accession:P06732
Molecular Weight:Calculated: 40kDaObserved: 43kDa
NCBI Full Name:Creatine kinase M-type
NCBI Synonym Full Names:creatine kinase, muscle
NCBI Official Symbol:CKM��
NCBI Official Synonym Symbols:CKMM; M-CK��
NCBI Protein Information:creatine kinase M-type; creatine kinase M chain
UniProt Protein Name:Creatine kinase M-type
UniProt Synonym Protein Names:Creatine kinase M chain; M-CKCreatine kinase M-type, N-terminally processed
Protein Family:Creatine kinase
UniProt Gene Name:CKM��
UniProt Entry Name:KCRM_HUMAN

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