The Human CKLF (Chemokine Like Factor) ELISA Kit allows for the quantitative assessment of CKLF levels in various samples of human origin. CKLF is a chemokine-like protein involved in immune responses and inflammation modulation. It plays a role in cell migration, differentiation, and activation, making it important in various physiological and pathological processes. Elevated CKLF levels can be indicative of inflammatory conditions or immune-related diseases, serving as a valuable biomarker for research and clinical investigations. Accurate measurement of CKLF is crucial for understanding its role in immune regulation and potential disease implications.
This ELISA kit offers high sensitivity and specificity, ensuring reliable and reproducible results. With stringent quality controls in manufacturing, the kit delivers robust performance and ease of use, making it suitable for diverse applications in both research and clinical settings. Rely on Assay Genie's CKLF ELISA Kit for precise and dependable quantification of this chemokine-like factor in your studies.
Product Name:
Human CKLF (Chemokine Like Factor) ELISA Kit
SKU:
AEES00084
Target:
Human CKLF (Chemokine Like Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CKLF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CKLF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CKLF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CKLF. You can calculate the concentration of Human CKLF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
89-99
89-102
Average (%)
95
96
96
1:4
Range (%)
85-94
101-110
87-97
Average (%)
91
105
91
1:8
Range (%)
96-108
90-101
93-104
Average (%)
101
95
100
1:16
Range (%)
93-104
97-109
86-98
Average (%)
100
103
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
96-108
100
Cell culture media (n=5)
95-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.36
24.54
85.14
8.85
23.55
87.14
Standard deviation
0.47
1.18
4.28
0.46
1.06
4.69
C V (%)
5.02
4.81
5.03
5.2
4.5
5.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CKLF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CKLF in samples. No significant cross-reactivity or interference between Human CKLF and analogues was observed.
