The Human CKB (Creatine Kinase Brain) ELISA Kit is a reliable and precise tool for the quantitative detection of CKB levels in human serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring accurate and consistent results for a variety of research needs.Creatine Kinase Brain, also known as CKB, is an enzyme primarily found in the brain and plays a critical role in energy metabolism. Abnormal levels of CKB have been associated with various neurological disorders, making it a valuable biomarker for studying brain health and potential therapeutic interventions.
With its high-quality components and user-friendly instructions, the Human CKB ELISA Kit is an indispensable tool for researchers studying brain function, neurological diseases, and energy metabolism. Trust in this kit for reliable and reproducible results in your research endeavors.
Product Name:
Human CKB (Creatine Kinase, Brain) ELISA Kit
SKU:
HUES03300
Target:
Human CKB (Creatine Kinase, Brain)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CKB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CKB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CKB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CKB. You can calculate the concentration of Human CKB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
100-114
97-109
Average (%)
100
106
103
1:4
Range (%)
88-99
85-100
80-92
Average (%)
93
92
87
1:8
Range (%)
92-106
79-90
87-99
Average (%)
99
85
94
1:16
Range (%)
88-103
82-94
84-95
Average (%)
94
87
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
93-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.02
3.18
7.58
1.03
3.11
8.09
Standard deviation
0.07
0.17
0.23
0.06
0.13
0.43
C V (%)
6.86
5.35
3.03
5.83
4.18
5.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CKB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CKB in samples. No significant cross-reactivity or interference between Human CKB and analogues was observed.
