Human CHRM2 (Cholinergic Receptor, Muscarinic 2) CLIA Kit
The Human CHRM2 (Cholinergic Receptor Muscarinic 2) CLIA Kit is specifically designed for the accurate detection of CHRM2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.CHRM2 is a key receptor involved in the cholinergic signaling pathway, playing a crucial role in neurotransmission and various physiological processes. Dysregulation of CHRM2 has been associated with neurological disorders, including Alzheimer's disease and schizophrenia, making it an important biomarker for studying these conditions and potentially developing new therapeutic approaches.
Overall, the Human CHRM2 CLIA Kit provides researchers with a valuable tool for investigating the role of CHRM2 in health and disease, with the potential to advance our understanding of the underlying mechanisms and pave the way for novel treatment strategies.
Product Name:
Human CHRM2 (Cholinergic Receptor, Muscarinic 2) CLIA Kit
SKU:
HUES00462
Target:
Human CHRM2 (Cholinergic Receptor, Muscarinic 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CHRM2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CHRM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CHRM2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CHRM2. You can calculate the concentration of Human CHRM2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
89-104
89-105
Average (%)
96
95
96
1:4
Range (%)
97-109
88-102
91-106
Average (%)
103
95
98
1:8
Range (%)
103-117
90-104
90-106
Average (%)
108
98
97
1:16
Range (%)
91-101
96-110
96-112
Average (%)
96
103
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
93
EDTA plasma (n=5)
89-104
97
Cell culture media (n=5)
90-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.31
314.22
992.6
101.6
319.8
990.66
Standard deviation
10.02
36.76
97.08
10.96
36.23
85.0
C V (%)
10.51
11.7
9.78
10.79
11.33
8.58
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CHRM2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CHRM2 in samples. No significant cross-reactivity or interference between Human CHRM2 and analogues was observed.
