Human Chorionic Somatomammotropin Hormone 1 (CSH1) ELISA Kit
The Human Chorionic Somatomammotropin Hormone-1 (CSH1) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of CSH1 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.CSH1, also known as placental lactogen, is a hormone produced by the placenta during pregnancy. It plays a crucial role in fetal development, maternal metabolism, and lactation. Abnormal levels of CSH1 have been associated with various complications during pregnancy, such as gestational diabetes and preeclampsia, making it an important biomarker for monitoring maternal and fetal health.
By using the CSH1 ELISA Kit, researchers can better understand the role of this hormone in pregnancy-related disorders and potentially develop new strategies for diagnosing and treating these conditions. Overall, this kit is a valuable tool for studying the physiological functions of CSH1 and its impact on pregnancy outcomes.
Product Name:
Human Chorionic Somatomammotropin Hormone 1 (CSH1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
