Human Cholesterol-25-Hydroxylase (CH25H) ELISA Kit
The Human Cholesterol 25-Hydroxylase (CH25H) ELISA Kit is designed for the precise detection of CH25H levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.CH25H is an important enzyme involved in the biosynthesis of 25-hydroxycholesterol, a key regulator of immune responses and inflammation. Dysregulation of CH25H has been linked to various diseases such as atherosclerosis, autoimmune disorders, and viral infections, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With its advanced technology and accurate performance, the Human CH25H ELISA Kit is an indispensable tool for researchers exploring the role of CH25H in health and disease. Order yours today and unlock new insights into the complex mechanisms of cholesterol metabolism and immune regulation.
Product Name:
Human Cholesterol-25-Hydroxylase (CH25H) ELISA Kit
Product Code:
HUDL00556
Size:
96 Assays
Target:
CH25H
Synonyms:
h25OH, Cholesterol 25-monooxygenase
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
