The Human CHIT1 (Chitotriosidase 1) ELISA Kit is designed for the accurate detection of chitotriosidase 1 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.Chitotriosidase 1 is an enzyme that plays a crucial role in the immune system, particularly in the breakdown of chitin, a component of fungal cell walls.
Elevated levels of chitotriosidase 1 have been linked to various diseases, including Gaucher disease, asthma, and sarcoidosis, making it a valuable biomarker for studying these conditions and potential therapies.The Human CHIT1 ELISA Kit provides researchers with a powerful tool for studying the role of chitotriosidase 1 in health and disease, with the potential to uncover new insights and therapeutic targets.
Product Name:
Human CHIT1 (Chitotriosidase-1) ELISA Kit
SKU:
HUES03591
Target:
Human CHIT1 (Chitotriosidase-1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CHIT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CHIT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CHIT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CHIT1. You can calculate the concentration of Human CHIT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
84-97
88-99
Average (%)
100
90
93
1:4
Range (%)
94-109
79-92
89-104
Average (%)
99
85
95
1:8
Range (%)
88-99
86-99
85-97
Average (%)
93
92
92
1:16
Range (%)
91-104
86-99
86-97
Average (%)
97
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
271.44
618.7
2056.62
261.7
605.5
1888.39
Standard deviation
16.53
36.44
64.37
13.29
30.52
90.27
C V (%)
6.09
5.89
3.13
5.08
5.04
4.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CHIT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CHIT1 in samples. No significant cross-reactivity or interference between Human CHIT1 and analogues was observed.
