Human CHGA Recombinant Protein (RPPB3169)
- SKU:
- RPPB3169
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P10645
Description
| Product Name: | Human CHGA Recombinant Protein |
| Product Code: | RPPB3169 |
| Size: | 10µg |
| Species: | Human |
| Target: | CHGA |
| Synonyms: | CGA, CHGA, Vasostatin-2, Pituitary secretory protein I, SP-I. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The CHGA protein contains 20mM MES buffer pH-6, 2mM EDTA, 0.1mM PMSF and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80.0% as determined by Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MLPVNSPMNK GDTEVMKCIV EVISDTLSKP SPMPVSQECF ETLRGDERIL SILRHQNLLK ELQDLALQGA KERAHQQKKH SGFEDELSEV LENQSSQAEL KEAVEEPSSK DVMEKREDSK EAEKSGEATD GARPQALPEP MQESKAEGNN QAPGEEEEEE EEATNTHPPA SLPSQKYPGP QAEGDSEGLS QGLVDREKGL SAEPGWQAKR EEEEEEEEEA EAGEEAVPEE EGPTVVLNPH PSLGYKEIRK GESRSEALAV DGAGKPGAEE AQDPEGKGEQ EHSQQKEEEE EMAVVPQGLF RGGKSGELEQ EEERLSKEWE DSKRWSKMDQ LAKELTAEKR LEGQEEEEDN RDSSMKLSFR ARAYGFRGPG PQLRRGWRPS SREDSLEAGL PLQVRGYPEE KKEEEGSANR RPEDQELESL SAIEAELEKV AHQLQALRRG |
Chromgranin-A is part of the neuroendocrine secretory protein family. CHGA is located in secretory vesicles of neurons and endocrine cells. Chromgranin-A is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. Chromgranin-A has numerous biological activities on some tissues and organs and exerts a large spectrum of homeostatic actions, including antifungal and antimicrobial effect, modulation of cell adhesion, and inhibition of parathyroid hormone secretion.
Recombinant Human CHGA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 460 amino acids (19-457 a.a) and having a molecular mass of 51.2kDa (Molecular weight on SDS-PAGE will appear higher). Chromgranin-A is fused to 21 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CHGA: a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells and is a precursor to multiple biologically active peptides including vasostatin, pancreastatin, and chromostatin. Binds calcium with a low-affinity. Vasostatin negatively regulates angiogenesis and has antibacterial activity against some Gram-positive bacteria. Pancreastatin inhibits glucose induced insulin release from the pancreas. Chromostatin inhibits catecholamine release from chromaffin cells. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Vesicle; Cell adhesion Chromosomal Location of Human Ortholog: 14q32 Cellular Component: extracellular space; perinuclear region of cytoplasm; secretory granule Biological Process: defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; mast cell activation; mast cell chemotaxis; mast cell cytokine production; mast cell degranulation; negative regulation of catecholamine secretion; negative regulation of vasodilation; organelle organization and biogenesis; positive regulation of cAMP metabolic process; regulation of blood pressure; regulation of the force of heart contraction |
| NCBI Summary: | The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
| UniProt Code: | P10645 |
| NCBI GenInfo Identifier: | 215274270 |
| NCBI Gene ID: | 1113 |
| NCBI Accession: | P10645.7 |
| UniProt Secondary Accession: | P10645,Q53FA8, Q6NR84, Q96E84, Q96GL7, Q9BQB5, B2R9E9 |
| UniProt Related Accession: | P10645 |
| Molecular Weight: | 50,688 Da |
| NCBI Full Name: | Chromogranin-A |
| NCBI Synonym Full Names: | chromogranin A |
| NCBI Official Symbol: | CHGA�� |
| NCBI Official Synonym Symbols: | CGA�� |
| NCBI Protein Information: | chromogranin-A |
| UniProt Protein Name: | Chromogranin-A |
| UniProt Synonym Protein Names: | SL21 |
| Protein Family: | Chromogranin |
| UniProt Gene Name: | CHGA�� |
| UniProt Entry Name: | CMGA_HUMAN |
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