The Human Chemerin CLIA Kit is a cutting-edge assay designed for the precise measurement of chemerin levels in human samples, including serum, plasma, and cell culture supernatants. This advanced kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Chemerin is a key protein involved in various physiological processes, including inflammation, adipogenesis, and immune response regulation. Dysregulation of chemerin levels has been associated with conditions such as obesity, diabetes, and inflammatory disorders, making it a valuable biomarker for studying these diseases and developing targeted therapies.
With its high-quality components and user-friendly protocol, the Human Chemerin CLIA Kit from Assay Genie is an essential tool for researchers looking to explore the role of chemerin in health and disease. Unlock new insights and advance your research with this innovative assay kit.
Product Name:
Human CHEM (Chemerin) CLIA Kit
SKU:
HUES00448
Target:
Human CHEM (Chemerin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CHEM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CHEM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CHEM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CHEM. You can calculate the concentration of Human CHEM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
85-97
92-109
Average (%)
95
91
100
1:4
Range (%)
97-114
89-103
91-104
Average (%)
104
94
97
1:8
Range (%)
88-99
103-117
96-106
Average (%)
93
109
101
1:16
Range (%)
100-116
98-110
100-118
Average (%)
108
104
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
95-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
90.96
236.44
670.99
94.29
243.16
604.29
Standard deviation
8.88
17.78
69.18
9.7
22.44
51.06
C V (%)
9.76
7.52
10.31
10.29
9.23
8.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CHEM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CHEM in samples. No significant cross-reactivity or interference between Human CHEM and analogues was observed.
