Human CGRP (Calcitonin Gene Related Peptide) ELISA Kit
The Human CGRP (Calcitonin Gene-Related Peptide) ELISA Kit is a reliable tool for detecting and measuring levels of CGRP in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reproducible results for various research applications.CGRP is a neuropeptide that plays a crucial role in processes like vasodilation, inflammation, and pain transmission.
It is implicated in various physiological and pathological conditions, including migraine, neurogenic inflammation, and cardiovascular disorders. By accurately measuring CGRP levels, researchers can better understand the role of this peptide in disease mechanisms and potential therapeutic interventions. Get your Human CGRP ELISA Kit now and unlock new insights into the functions and implications of CGRP in health and disease.
Product Name:
Human CGRP (Calcitonin Gene Related Peptide) ELISA Kit
SKU:
HUES01782
Target:
Human CGRP (Calcitonin Gene Related Peptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CGRP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CGRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CGRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CGRP1. You can calculate the concentration of Human CGRP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-98
89-102
94-107
Average (%)
90
94
101
1:4
Range (%)
86-100
84-95
89-102
Average (%)
92
89
94
1:8
Range (%)
90-103
84-99
89-102
Average (%)
96
91
95
1:16
Range (%)
92-106
86-97
87-98
Average (%)
98
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
91-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.15
145.75
349.43
51.54
133.87
361.48
Standard deviation
3.1
8.31
13.66
3.4
6.84
13.59
C V (%)
6.44
5.7
3.91
6.6
5.11
3.76
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CGRP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CGRP1 in samples. No significant cross-reactivity or interference between Human CGRP1 and analogues was observed.
