Human CGβ (Chorionic Gonadotrophin Beta) ELISA Kit
The CGβ ELISA Kit is designed for the quantitative detection of Chorionic Gonadotrophin Beta (CGβ) levels in various human biological samples. CGβ is a subunit of human chorionic gonadotropin (hCG) and is involved in the early stages of pregnancy. It plays a crucial role in supporting the implantation and nurturing of the embryo in the uterus. Accurate measurement of CGβ is essential for monitoring pregnancy status, particularly in diagnosing and managing pregnancy-related complications.
Our CGβ ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under strict quality control standards, this kit provides robust performance and is user-friendly, making it an excellent tool for both clinical and research applications. Trust our CGβ ELISA Kit for accurate and dependable quantification of this vital biomarker in your studies and clinical assessments.
Product Name:
Human CGβ (Chorionic Gonadotrophin Beta) ELISA Kit
SKU:
AEES00040
Target:
Human CGβ (Chorionic Gonadotrophin Beta)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 mIU/mL
Detection range:
15.63-1000 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CGβ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CGβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CGβ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CGβ. You can calculate the concentration of Human CGβ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-103
92-106
91-105
Average (%)
100
99
97
1:4
Range (%)
85-94
93-105
98-112
Average (%)
91
101
104
1:8
Range (%)
93-104
86-100
87-96
Average (%)
96
93
93
1:16
Range (%)
90-101
85-96
97-111
Average (%)
97
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-100
94
EDTA plasma (n=5)
90-100
95
Cell culture media (n=5)
93-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.39
109.68
450.13
43.13
113.96
439.91
Standard deviation
2.06
5.47
18.19
2.32
5.64
22.88
C V (%)
5.1
4.99
4.04
5.38
4.95
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CGβ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CGβ in samples. No significant cross-reactivity or interference between Human CGβ and analogues was observed.
