The Human CFI (Complement Factor I) ELISA Kit is a specialized assay designed for the quantitative detection of CFI levels in various biological samples. Complement Factor I is a vital regulator of the complement system, playing a key role in the modulation of complement activation, immune responses, and host defense mechanisms. As a pivotal inhibitor, CFI contributes to maintaining immune homeostasis, preventing excessive complement activation, and regulating inflammation. Understanding CFI levels is crucial for investigating the complement system's function, immune responses, and inflammatory processes influenced by complement activation and regulation.
Accurate measurement of CFI is essential for deciphering its regulatory functions in complement-mediated pathways, immune modulation, and disease pathogenesis. With exceptional sensitivity and specificity, Assay Genie's CFI ELISA Kit ensures precise and reproducible results. Manufactured under stringent quality standards, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for both scientific research and clinical applications. Researchers can rely on Assay Genie's CFI ELISA Kit for dependable quantification of this critical complement factor in their studies.
Product Name:
Human CFI (Complement Factor I) ELISA Kit
SKU:
AEES00094
Target:
Human CFI (Complement Factor I)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CFI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CFI. You can calculate the concentration of Human CFI in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-103
95-107
97-113
Average (%)
97
102
105
1:4
Range (%)
91-100
88-102
92-99
Average (%)
94
94
96
1:8
Range (%)
93-104
91-105
88-98
Average (%)
100
98
93
1:16
Range (%)
99-108
90-99
98-104
Average (%)
104
93
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-111
104
EDTA plasma (n=5)
91-103
98
Cell culture media (n=5)
90-102
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
8.43
20.17
94.33
8.12
21.37
82.88
Standard deviation
0.47
1.01
4.48
0.47
0.99
3.56
C V (%)
5.58
5.01
4.75
5.79
4.63
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CFI concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CFI in samples. No significant cross-reactivity or interference between Human CFI and analogues was observed.
