The Human CFH (Complement Factor H) ELISA Kit is a specialized assay designed for the precise quantitative detection of CFH levels in various biological samples. Complement Factor H is a key regulator of the alternative complement pathway, crucial for immune homeostasis, host defense, and preventing excessive complement activation. CFH serves as a vital factor in protecting host cells from complement-mediated damage, modulation of complement activity, and regulation of inflammation. Understanding CFH levels is pivotal for unraveling immune responses, complement system function, and pathogenic mechanisms related to complement dysregulation.
Accurate measurement of CFH is essential for investigating its regulatory roles in immune responses, inflammatory processes, and complement-mediated diseases. With outstanding sensitivity and specificity, Assay Genie's CFH ELISA Kit ensures reliable and reproducible results. Manufactured under stringent quality controls, this kit offers robust performance and user-friendly protocols, making it an excellent choice for both basic research and clinical studies. Researchers can trust Assay Genie's CFH ELISA Kit for precise quantification of this critical complement factor in their investigations.
Product Name:
Human CFH (Complement Factor H) ELISA Kit
SKU:
AEES00093
Target:
Human CFH (Complement Factor H)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CFH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CFH. You can calculate the concentration of Human CFH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
88-101
89-99
Average (%)
104
96
94
1:4
Range (%)
95-109
87-100
85-96
Average (%)
103
95
92
1:8
Range (%)
90-98
96-111
96-107
Average (%)
94
103
99
1:16
Range (%)
98-107
92-102
86-97
Average (%)
104
95
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-94
91
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
101-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.18
22.01
96.03
9.61
24.44
89.89
Standard deviation
0.53
1.03
4.38
0.57
1.22
4.54
C V (%)
5.77
4.68
4.56
5.93
4.99
5.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CFH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CFH in samples. No significant cross-reactivity or interference between Human CFH and analogues was observed.
