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Human Centromere Protein F (CENPF) ELISA Kit

SKU:
HUDL00535
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P49454
Sensitivity:
0.046ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CENP-F,CENF,Hcp-1,Centromere protein F(350/400kD,Mitosin),AH antigen,Kinetochore protein CENPF,Mitosin
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Centromere Protein F (CENPF) ELISA Kit

The Human Centromere Protein F (CENPF) ELISA Kit is specifically designed for the accurate measurement of CENPF levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.CENPF is a key protein involved in centromere assembly and regulation during cell division, making it vital for proper chromosome segregation.

Dysregulation of CENPF has been linked to various diseases, including cancer and genetic disorders, highlighting its importance as a biomarker for studying these conditions and developing potential treatments.Overall, the Human Centromere Protein F (CENPF) ELISA Kit is a valuable tool for researchers seeking to investigate the role of CENPF in cellular processes and disease pathogenesis.

Product Name:Human Centromere Protein F (CENPF) ELISA Kit
Product Code:HUDL00535
Size:96 Assays
Target:CENPF
Synonyms:CENP-F, CENF, Hcp-1, Centromere protein F(350/400kD,Mitosin), AH antigen, Kinetochore protein CENPF, Mitosin
Detection Method:Sandwich
Range:0.156-10 ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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