The Human CENPF (Centromere Protein F) CLIA Kit is specifically designed for the precise measurement of CENPF levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.CENPF is a key protein involved in the regulation of cell division and chromosome segregation during cell cycle progression. Its dysregulation has been linked to various diseases, including cancer and genetic disorders, making it a valuable biomarker for understanding these conditions and potentially developing targeted treatments.
With its reliable performance and versatility, the Human CENPF CLIA Kit is an essential tool for researchers studying cell biology, cancer, and other conditions where CENPF plays a critical role. Trust this kit to deliver precise and reliable results for your research needs.
Product Name:
Human CENPF (Centromere Protein F) CLIA Kit
SKU:
HUES00461
Target:
Human CENPF (Centromere Protein F)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CENPF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CENPF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CENPF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CENPF. You can calculate the concentration of Human CENPF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
92-103
88-100
Average (%)
93
98
93
1:4
Range (%)
99-118
100-112
103-117
Average (%)
108
106
109
1:8
Range (%)
97-113
92-107
93-108
Average (%)
105
100
101
1:16
Range (%)
91-103
89-104
88-101
Average (%)
97
96
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
97-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.32
173.87
852.37
106.18
169.95
921.68
Standard deviation
12.43
13.13
59.07
11.38
14.24
60.55
C V (%)
11.8
7.55
6.93
10.72
8.38
6.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CENPF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CENPF in samples. No significant cross-reactivity or interference between Human CENPF and analogues was observed.
