The Human CEA (Carcinoembryonic Antigen) ELISA Kit is designed for the quantitative measurement of CEA levels in various biological samples of human origin. CEA is a glycoprotein that serves as a tumor marker, particularly associated with gastrointestinal cancers such as colorectal carcinoma. Elevations in CEA levels can indicate disease progression, making it a valuable biomarker for cancer diagnosis, monitoring therapeutic responses, and detecting cancer recurrence. Accurate detection and quantification of CEA are essential for oncological research, clinical diagnostics, and patient management.
This ELISA kit offers exceptional specificity and sensitivity, ensuring precise and reproducible results. Manufactured under stringent quality control measures, the kit provides reliable performance and user-friendly protocols. Choose Assay Genie's CEA ELISA Kit for robust and dependable quantification of this significant tumor marker in your studies and clinical applications.
Product Name:
Human CEA (Carcinoembryonic Antigen) ELISA Kit
SKU:
AEES00083
Target:
Human CEA (Carcinoembryonic Antigen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CEA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CEA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CEA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CEA. You can calculate the concentration of Human CEA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-107
91-101
96-112
Average (%)
104
98
104
1:4
Range (%)
87-96
85-94
87-99
Average (%)
90
90
93
1:8
Range (%)
100-110
91-106
93-105
Average (%)
103
98
101
1:16
Range (%)
89-96
95-108
101-109
Average (%)
93
103
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
96
EDTA plasma (n=5)
93-103
96
Cell culture media (n=5)
92-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.2
4.32
0.5
1.04
4.59
Standard deviation
0.03
0.06
0.18
0.03
0.05
0.19
C V (%)
6.12
5.0
4.17
6.0
4.81
4.14
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CEA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CEA in samples. No significant cross-reactivity or interference between Human CEA and analogues was observed.
