Human CDNF Recombinant Protein (RPPB0123)
- SKU:
- RPPB0123
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q49AH0
- Research Area:
- Neurotrophins
Description
| Product Name: | Human CDNF Recombinant Protein |
| Product Code: | RPPB0123 |
| Size: | 20µg |
| Species: | Human |
| Target: | CDNF |
| Synonyms: | Cerebral neurotrophic factor, arginine-rich, mutated in early stage tumors-like 1, Conserved neurotrophic factor, ARMET-like protein 1, ARMETL1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | CDNF protein was lyophilized from a 0.2�m filtered concentrated solution in 1xPBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized�CDNF in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized CDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CDNF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 96.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | QEAGGRPGAD CEVCKEFLNR FYKSLIDRGV NFSLDTIEKE LISFCLDTKG KENRLCYYLG ATKDAATKIL SEVTRPMSVH MPAMKICEKL KKLDSQICEL KYEKTLDLAS VDLRKMRVAE LKQILHSWGE ECRACAEKTD YVNLIQELAP KYAATHPKTE L |
| Biological Activity: | The ED50 as determined by its ability to stimulate the proliferation of rat C6 cells is 15-25�g/ml, corresponding to a specific activity of 40-67units/mg. |
CDNF is a member of the ARMET family and acts as a trophic factor for neurons. CDNF inhibits the (6-OHDA)-induced degeneration of neurons. When CDNF controlled after 6-OHDA-lesioning, it reestablishes the function and inhibits the degeneration of neurons in substantia nigra. CDNF is universally expressed in neuronal and non-neuronal tissues. The highest levels in the brain are found in the optic nerve and corpus callosum.
CDNF Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18.5kDa.The CDNF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ARMETL1: Trophic factor for dopamine neurons. Prevents the 6- hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra. Belongs to the ARMET family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 10p13 Cellular Component: extracellular region Molecular Function:growth factor activity |
| UniProt Code: | Q49AH0 |
| NCBI GenInfo Identifier: | 143955278 |
| NCBI Gene ID: | 441549 |
| NCBI Accession: | Q49AH0.2 |
| UniProt Related Accession: | Q49AH0 |
| Molecular Weight: | |
| NCBI Full Name: | Cerebral dopamine neurotrophic factor |
| NCBI Synonym Full Names: | cerebral dopamine neurotrophic factor |
| NCBI Official Symbol: | CDNF�� |
| NCBI Official Synonym Symbols: | ARMETL1�� |
| NCBI Protein Information: | cerebral dopamine neurotrophic factor |
| UniProt Protein Name: | Cerebral dopamine neurotrophic factor |
| UniProt Synonym Protein Names: | ARMET-like protein 1; Conserved dopamine neurotrophic factor |
| Protein Family: | Cerebral dopamine neurotrophic factor |
| UniProt Gene Name: | CDNF�� |
| UniProt Entry Name: | CDNF_HUMAN |
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