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Human CDK2AP1 Recombinant Protein (RPPB2455)

SKU:
RPPB2455
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O14519
Research Area:
Enzymes
$371
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Description

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Product Name:Human CDK2AP1 Recombinant Protein
Product Code:RPPB2455
Size:25µg
Species:Human
Target:CDK2AP1
Synonyms:Cyclin-dependent kinase 2-associated protein 1, CDK2-associated protein 1, Putative oral cancer suppressor, Deleted in oral cancer 1, DOC-1, CDK2AP1, CDKAP1, DOC1, ST19, DORC1, p12DOC-1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear colorless solution.
Formulation:The CDK2AP1 solution contains 20mM Tris-HCl pH-8, 0.1M NaCl and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:RGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSY KPNLAAHMPA AALNAAGSVH SPSTSMATSS QYRQLLSDYG PPSLGYTQGT GNSQVPQSKY AELLAIIEEL GKEIRPTYAG SKSAMERLKR GIIHARGLVR ECLAETERNA RS

CDK2AP1 is a CDK2-associated protein that negatively regulates CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. CDK2AP1 interacts with DNA polymerase alpha and mediates the phosphorylation of the large p180 subunit, which implicates the regulatory role in DNA replication during S phase of the cell cycle.

CDK2AP1 Human Recombinant fused to 37 amino acid His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 152 amino acids (1-115 a.a.) and having a molecular mass of 16.6 kDa. The CDK2AP1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:CDK2AP1: specific inhibitor of the cell-cycle kinase CDK2. Belongs to the CDK2AP family.
UniProt Protein Details:

Protein type:DNA replication; Tumor suppressor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: perinuclear region of cytoplasm; nucleus

Biological Process: positive regulation of protein amino acid phosphorylation; DNA-dependent DNA replication; cell cycle

NCBI Summary:The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]
UniProt Code:O14519
NCBI GenInfo Identifier:6014988
NCBI Gene ID:8099
NCBI Accession:O14519.1
UniProt Secondary Accession:O14519,F5GYA4,
UniProt Related Accession:O14519
Molecular Weight:115
NCBI Full Name:Cyclin-dependent kinase 2-associated protein 1
NCBI Synonym Full Names:cyclin-dependent kinase 2 associated protein 1
NCBI Official Symbol:CDK2AP1��
NCBI Official Synonym Symbols:DOC1; ST19; DORC1; doc-1; p12DOC-1��
NCBI Protein Information:cyclin-dependent kinase 2-associated protein 1; Deleted in oral cancer-1; deleted in oral cancer 1; CDK2-associated protein 1; putative oral cancer suppressor
UniProt Protein Name:Cyclin-dependent kinase 2-associated protein 1
UniProt Synonym Protein Names:Deleted in oral cancer 1; DOC-1; Putative oral cancer suppressor
Protein Family:Cyclin-dependent kinase 2-associated protein
UniProt Gene Name:CDK2AP1��
UniProt Entry Name:CDKA1_HUMAN

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