Human CDCA8 Recombinant Protein (RPPB3121)
- SKU:
- RPPB3121
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q53HL2
Description
| Product Name: | Human CDCA8 Recombinant Protein |
| Product Code: | RPPB3121 |
| Size: | 10µg |
| Species: | Human |
| Target: | CDCA8 |
| Synonyms: | Cell division cycle associated 8, BOR, hDasraB, FLJ12042, MESRGP, Pluripotent embryonic stem cell-related gene 3 protein, PESCRG3, BOREALIN. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The CDCA8 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 50% glycerol, 1mM DTT, 1mM EDTA and 0.2M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MRKLASFLKD FDREVEIRIK QIESDRQNLL KEVDNLYNIE ILRLPKALRE MNWLDYFALG GNKQALEEAA TADLDITEIN KLTAEAIQTP LKSAKTRKVI QVDEMIVEEE EEEENERKNL QTARVKRCPP SKKRTQSIQG KGKGKRSSRA NTVTPAVGRLEVSMVKPTPG LTPRFDSRVF KTPGLRTPAA GERIYNISGN GSPLADSKEI FLTVPVGGGE SLRLLASDLQ RHSIAQLDPE ALGNIKKLSN RLAQICSSIR THK |
CDCA8 is a component of the chromosomal passenger complex (CPC). The CPC complex has crucial functions at the centromere in ensuring accurate chromosome placement and isolation and is obligatory for chromatin-induced microtubule stabilization and spindle assembly. In the complex, CDCA8 protein is needed to direct the CPC to centromeric DNA. Removal of CDCA8 delays mitotic progression and causes kinetochore-spindle to mis-attach.
CDCA8 Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 283 amino acids (19-280a.a.) and having a molecular mass of 31.6kDa. The CDCA8 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Borealin: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Belongs to the borealin family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Nucleolus Chromosomal Location of Human Ortholog: 1p34.3 Cellular Component: intercellular bridge; protein complex; nucleolus; spindle; midbody; nucleus; cytosol; chromosome, pericentric region; chromocenter Molecular Function:protein binding Biological Process: cell division; mitotic cell cycle; mitotic metaphase plate congression; chromosome organization and biogenesis |
| NCBI Summary: | This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013] |
| UniProt Code: | Q53HL2 |
| NCBI GenInfo Identifier: | 110832774 |
| NCBI Gene ID: | 55143 |
| NCBI Accession: | Q53HL2.2 |
| UniProt Secondary Accession: | Q53HL2,Q53HN1, Q96AM3, Q9NVW5, D3DPT4, |
| UniProt Related Accession: | Q53HL2 |
| Molecular Weight: | 280 |
| NCBI Full Name: | Borealin |
| NCBI Synonym Full Names: | cell division cycle associated 8 |
| NCBI Official Symbol: | CDCA8�� |
| NCBI Official Synonym Symbols: | BOR; DasraB; MESRGP; BOREALIN�� |
| NCBI Protein Information: | borealin; Dasra B; dasra-B; hDasra-B; cell division cycle-associated protein 8; pluripotent embryonic stem cell-related gene 3 protein |
| UniProt Protein Name: | Borealin |
| UniProt Synonym Protein Names: | Cell division cycle-associated protein 8; Dasra-B; hDasra-B; Pluripotent embryonic stem cell-related gene 3 protein |
| UniProt Gene Name: | CDCA8�� |
| UniProt Entry Name: | BOREA_HUMAN |
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