Human CD99 (Cluster of Differentiation 99) CLIA Kit
Utilizing a sandwich enzyme-linked immunosorbent assay (ELISA) methodology, the Human CD99 (Cluster of Differentiation 99) CLIA Kit from Assay Genie allows for the precise and efficient measurement of CD99 levels in human samples. This kit boasts excellent sensitivity and specificity, ensuring accurate and consistent results for various research purposes.CD99, a cell surface glycoprotein, is involved in numerous cellular processes, including cell adhesion, migration, and apoptosis. Dysregulation of CD99 expression has been linked to various diseases, including certain cancers and autoimmune disorders.
By quantifying CD99 levels in biological samples, researchers can gain valuable insights into disease mechanisms and potentially identify novel therapeutic targets.Perfect for investigating the role of CD99 in health and disease, the Human CD99 CLIA Kit provides researchers with a powerful tool for advancing their studies in immunology, oncology, and beyond. Trust Assay Genie's expertise in assay development to enhance the accuracy and reliability of your research findings.
Product Name:
Human CD99 (Cluster of Differentiation 99) CLIA Kit
SKU:
HUES00470
Target:
Human CD99 (Cluster of Differentiation 99)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CD99. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD99 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD99, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CD99. You can calculate the concentration of Human CD99 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-113
101-118
90-103
Average (%)
107
108
95
1:4
Range (%)
101-112
99-115
97-108
Average (%)
106
105
103
1:8
Range (%)
102-118
85-99
95-110
Average (%)
109
90
102
1:16
Range (%)
88-102
99-115
96-109
Average (%)
95
105
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
96
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.99
180.43
878.54
100.27
182.93
954.3
Standard deviation
13.08
19.31
70.55
11.69
21.92
107.93
C V (%)
12.23
10.7
8.03
11.66
11.98
11.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CD99 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CD99 in samples. No significant cross-reactivity or interference between Human CD99 and analogues was observed.
