Human CD8A Recombinant Protein (RPPB3027)
- SKU:
- RPPB3027
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P01732
- Research Area:
- CD Antigens
Description
| Product Name: | Human CD8A Recombinant Protein |
| Product Code: | RPPB3027 |
| Size: | 10µg |
| Species: | Human |
| Target: | CD8A |
| Synonyms: | CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, MAL, CD8, Leu2, p32, T-cell surface glycoprotein CD8 alpha chain isoform 1. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The CD8A solution (0.5mg/ml) contains Phosphate-Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPSQFRVSP LDRTWNLGET VELKCQVLLS NPTSGCSWLF QPRGAAASPT FLLYLSQNKPKAAEGLDTQR FSGKRLGDTF VLTLSDFRRE NEGYYFCSAL SNSIMYFSHF VPVFLPAKPTTTPAPRPPTP APTIASQPLS LRPEACRPAA GGAVHTRGLD FACDHHHHHH |
CD8 protein is a glycoprotein that is located on the cell surface of cytotoxic�T lymphocytes the interferes interactions between cells of the immune system. This protein is a coreceptor that helps detect antigens shown by the APC (antigen presenting cells) as part of the class I MHC proteins. It can be a homodimer build from 2 alpha chains orheterodimer build from alpha and beta chains.
CD8A Human Recombinant produced in Baculovirus is a single, glycosylated, polypeptide chain containing 170 amino acids (22-182aa) and having a molecular mass of 18.7kDa.CD8A is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. |
| UniProt Protein Details: | Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9 Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not By similarity. Involvement in Disease: CD8 deficiency, familial (CD8 deficiency) [MIM:608957]: An immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry. Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| NCBI Summary: | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | P01732 |
| NCBI GenInfo Identifier: | 116035 |
| NCBI Gene ID: | 925 |
| NCBI Accession: | P01732.1 |
| UniProt Secondary Accession: | P01732,Q13970, Q4ZG17, D6W5M8, |
| UniProt Related Accession: | P01732 |
| Molecular Weight: | |
| NCBI Full Name: | T-cell surface glycoprotein CD8 alpha chain |
| NCBI Synonym Full Names: | CD8a molecule |
| NCBI Official Symbol: | CD8A�� |
| NCBI Official Synonym Symbols: | CD8; MAL; p32; Leu2�� |
| NCBI Protein Information: | T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2 |
| UniProt Protein Name: | T-cell surface glycoprotein CD8 alpha chain |
| UniProt Synonym Protein Names: | T-lymphocyte differentiation antigen T8/Leu-2 |
| Protein Family: | CD81 antigen |
| UniProt Gene Name: | CD8A�� |
| UniProt Entry Name: | CD8A_HUMAN |
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