Human CD55 Recombinant Protein (RPPB3079)
- SKU:
- RPPB3079
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P08174
- Research Area:
- CD Antigens
Description
| Product Name: | Human CD55 Recombinant Protein |
| Product Code: | RPPB3079 |
| Size: | 10µg |
| Species: | Human |
| Target: | CD55 |
| Synonyms: | CD55 Antigen, DAF, CD55 Molecule, CHAPLE, Cromer Blood Group Antigen, Complement Decay-Accelerating Factor, CROM. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | CD55protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10%glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPDCGLPPD VPNAQPALEG RTSFPEDTVI TYKCEESFVK IPGEKDSVIC LKGSQWSDIE EFCNRSCEVP TRLNSASLKQ PYITQNYFPV GTVVEYECRP GYRREPSLSP KLTCLQNLKW STAVEFCKKK SCPNPGEIRN GQIDVPGGIL FGATISFSCN TGYKLFGSTS SFCLISGSSV QWSDPLPECR EIYCPAPPQI DNGIIQGERD HYGYRQSVTY ACNKGFTMIG EHSIYCTVNN DEGEWSGPPP ECRGKSLTSK VPPTVQKPTT VNVPTTEVSP TSQKTTTKTT TPNAQATRST PVSRTTKHFH ETTPNKGSGT TSHHHHHH |
Decay-Accelerating Factor Isoform 1 or CD55, is a protein that attached to the cell membrane through glycophosphatidylinositol (GPI) anchor. CD55 regulates the complement system in the outer membrane. The protein is a receptor to many types of enteroviruses such as coxsackieviruses. CD55 is widely scattered amidst hematopoietic & non-hematopoietic cells. The CD55 has a crucial part in tumorigenesis, autocrine loops for cell rescue and evasion of apoptosis, cell motility, invasiveness etc.
CD55 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (35-353 a.a.) and fused to a 9 aa His Tag at C-terminus containing a total of 328 amino acids and having a molecular mass of 36kDa. CD55 shows multiple bands between 40-57kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor; Cell surface Chromosomal Location of Human Ortholog: 1q32 Cellular Component: cell surface; integral to plasma membrane; extracellular region; plasma membrane; lipid raft Molecular Function:viral receptor activity; protein binding; lipid binding Biological Process: respiratory burst; entry of virus into host cell; elevation of cytosolic calcium ion concentration; negative regulation of complement activation; regulation of complement activation; innate immune response; regulation of lipopolysaccharide-mediated signaling pathway; complement activation, classical pathway Disease: Blood Group, Cromer System |
| NCBI Summary: | This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P08174 |
| NCBI GenInfo Identifier: | 10835143 |
| NCBI Gene ID: | 1604 |
| NCBI Accession: | NP_000565.1 |
| UniProt Secondary Accession: | P08174,P09679, P78361, B1AP14, D3DT83, D3DT84, |
| UniProt Related Accession: | P08174 |
| Molecular Weight: | 41,400 Da |
| NCBI Full Name: | complement decay-accelerating factor isoform 1 preproprotein |
| NCBI Synonym Full Names: | CD55 molecule, decay accelerating factor for complement (Cromer blood group) |
| NCBI Official Symbol: | CD55�� |
| NCBI Official Synonym Symbols: | CR; TC; DAF; CROM�� |
| NCBI Protein Information: | complement decay-accelerating factor; CD55 antigen |
| UniProt Protein Name: | Complement decay-accelerating factor |
| Protein Family: | Complement decay-accelerating factor |
| UniProt Gene Name: | CD55�� |
| UniProt Entry Name: | DAF_HUMAN |
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