The Human CD4 (Cluster of Differentiation 4) CLIA Kit is a highly reliable and accurate assay designed for the quantitative detection of CD4 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.CD4 is a key protein that serves as a marker for T helper cells, playing a crucial role in the immune system's response to pathogens and infections. Monitoring CD4 levels is essential for assessing immune function, particularly in conditions such as HIV/AIDS where CD4 counts are used to determine disease progression and treatment efficacy.
With its high performance and ease of use, the Human CD4 CLIA Kit is an invaluable tool for researchers studying immunology, infectious diseases, and autoimmune disorders. By accurately measuring CD4 levels, this kit enables in-depth investigations into immune responses and the development of targeted therapies for various immune-related conditions.
Product Name:
Human CD4 (Cluster of Differentiation 4) CLIA Kit
SKU:
HUES00471
Target:
Human CD4 (Cluster of Differentiation 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.56 ng/mL
Detection range:
0.94-60 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CD4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CD4. You can calculate the concentration of Human CD4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-104
88-99
94-107
Average (%)
95
93
102
1:4
Range (%)
92-104
87-98
94-110
Average (%)
97
92
101
1:8
Range (%)
88-103
98-111
87-98
Average (%)
95
105
93
1:16
Range (%)
102-118
90-102
101-116
Average (%)
109
95
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
91
EDTA plasma (n=5)
85-100
92
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.31
5.61
26.06
3.57
5.71
24.07
Standard deviation
0.33
0.62
2.56
0.33
0.52
2.48
C V (%)
9.97
11.05
9.82
9.24
9.11
10.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CD4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CD4 in samples. No significant cross-reactivity or interference between Human CD4 and analogues was observed.
