The Human CD235 Monoclonal Antibody (HIR2-AGEL0302) offered by Assay Genie is a cutting-edge tool for researchers studying CD235, a key cell surface marker involved in blood cell development and blood group typing. This monoclonal antibody, optimized for use in human samples, is rigorously validated for various applications, including flow cytometry and immunohistochemistry.CD235, also known as glycophorin A, is a critical membrane protein found on red blood cells and erythroid precursors, playing a vital role in blood group antigen expression and structural integrity of red blood cells. Research on CD235 is instrumental in understanding the mechanisms of blood cell differentiation and blood group antigen diversity, with implications for transfusion medicine and genetic disorders related to blood type expression.
By utilizing the Human CD235 Monoclonal Antibody from Assay Genie, researchers gain a reliable and specific tool for investigating CD235 expression levels, distribution patterns, and functional roles in various experimental settings. This antibody facilitates detailed analysis of CD235 in different cell types and tissues, providing valuable insights into hematopoiesis, blood disorders, and transfusion compatibility. Unlock the potential of CD235 research with this high-quality monoclonal antibody from Assay Genie.
Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.