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Human CD200 Receptor 2 (CD200R2) ELISA Kit

SKU:
HUDL00483
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q6Q8B3
Sensitivity:
0.34ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
CD200R1L,CD200RLa,HuCD200R2,Cell surface glycoprotein CD200 receptor 2,CD200 cell surface glycoprotein receptor-like a,Cell surface glycoprotein OX2 receptor 2
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Human CD200 Receptor 2 (CD200R2) ELISA Kit

The Human CD200 Receptor 2 (CD200R2) ELISA Kit is a powerful tool for accurately measuring levels of CD200R2 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and consistent results, making it well-suited for a variety of research applications.CD200R2 is a key protein involved in immune regulation and inflammatory responses.

By studying CD200R2 levels, researchers can gain valuable insights into conditions such as autoimmune diseases, allergies, and cancer. This ELISA kit is instrumental in unraveling the role of CD200R2 in various diseases and in the development of potential therapeutic strategies. Trust the Human CD200 Receptor 2 (CD200R2) ELISA Kit for accurate and reliable detection of CD200R2 levels in human samples.

Product Name:Human CD200 Receptor 2 (CD200R2) ELISA Kit
Product Code:HUDL00483
Size:96 Assays
Target:CD200R2
Synonyms:CD200R1L, CD200RLa, HuCD200R2, Cell surface glycoprotein CD200 receptor 2, CD200 cell surface glycoprotein receptor-like a, Cell surface glycoprotein OX2 receptor 2
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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