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Human CD200 Receptor 1 (CD200R1) ELISA Kit

SKU:
HUDL00482
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8TD46
Sensitivity:
0.55ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
OX2R,HCRTR2,CD200R,MOX2R,MOX2 Receptor,Cell surface glycoprotein CD200 receptor 1,CD200 cell surface glycoprotein receptor,Cell surface glycoprotein OX2 receptor 1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Human CD200 Receptor 1 (CD200R1) ELISA Kit

The Human CD200 Receptor 1 (CD200R1) ELISA Kit from Assay Genie is a reliable tool for detecting CD200R1 levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures accurate and reproducible results, making it ideal for various research applications.CD200R1 is a critical receptor involved in regulating immune responses and is known to play a role in autoimmune diseases, cancer, and neuroinflammatory disorders.

By measuring CD200R1 levels, researchers can gain valuable insights into these conditions and potentially develop new therapeutic approaches.Overall, the Human CD200 Receptor 1 (CD200R1) ELISA Kit is a valuable resource for studying the role of CD200R1 in health and disease, offering researchers a powerful tool for advancing their research.

Product Name:Human CD200 Receptor 1 (CD200R1) ELISA Kit
Product Code:HUDL00482
Size:96 Assays
Target:CD200R1
Synonyms:Human CD200 Receptor 1 (CD200R1) ELISA Kit CD200R1 OX2R HCRTR2 CD200R MOX2R MOX2 Receptor Cell surface glycoprotein CD200 receptor 1 CD200 cell surface glycoprotein receptor Cell surface glycoprotein OX2 receptor 1 DL-CD200R1-Hu
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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