Human CD14 Recombinant Protein (RPPB3006)
- SKU:
- RPPB3006
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P08571
- Research Area:
- CD Antigens
Description
| Product Name: | Human CD14 Recombinant Protein |
| Product Code: | RPPB3006 |
| Size: | 10µg |
| Species: | Human |
| Target: | CD14 |
| Synonyms: | Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | CD14 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 30% glycerol 0.15M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSTTPEPCE LDDEDFRCVC NFSEPQPDWS EAFQCVSAVE VEIHAGGLNL EPFLKRVDAD ADPRQYADTV KALRVRRLTV GAAQVPAQLL VGALRVLAYS RLKELTLEDL KITGTMPPLP LEATGLALSS LRLRNVSWAT GRSWLAELQQ WLKPGLKVLS IAQAHSPAFS CEQVRAFPAL TSLDLSDNPG LGERGLMAAL CPHKFPAIQN LALRNTGMET PTGVCAALAA AGVQPHSLDL SHNSLRATVN PSAPRCMWSS ALNSLNLSFA GLEQVPKGLP AKLRVLDLSC NRLNRAPQPD ELPEVDNLTL DGNPFLVPGT ALPHEGSMNS GVV |
CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.
CD14 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 353 amino acids (20-349 a.a) and having a molecular mass of 37.9kDa. CD14is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor Cellular Component: anchored to external side of plasma membrane; extracellular space; cell surface; membrane; plasma membrane; lipopolysaccharide receptor complex; lipid raft; external side of plasma membrane Molecular Function:lipopolysaccharide binding Biological Process: response to bacterium; immune system process; response to molecule of bacterial origin; innate immune response; positive regulation of endocytosis; positive regulation of tumor necrosis factor production; inflammatory response; positive regulation of cytokine secretion |
| NCBI Summary: | This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014] |
| UniProt Code: | P08571 |
| NCBI GenInfo Identifier: | 6753332 |
| NCBI Gene ID: | 12475 |
| NCBI Accession: | NP_033971.1 |
| UniProt Related Accession: | P10810 |
| Molecular Weight: | |
| NCBI Full Name: | monocyte differentiation antigen CD14 |
| NCBI Synonym Full Names: | CD14 antigen |
| NCBI Official Symbol: | Cd14�� |
| NCBI Protein Information: | monocyte differentiation antigen CD14 |
| UniProt Protein Name: | Monocyte differentiation antigen CD14 |
| UniProt Synonym Protein Names: | Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14 |
| Protein Family: | Monocyte differentiation antigen |
| UniProt Gene Name: | Cd14�� |
| UniProt Entry Name: | CD14_MOUSE |
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