Human Chemokine C-C-Motif Receptor 1 (CCR1) ELISA Kit
The Human Chemokine C-C Motif Receptor 1 (CCR1) ELISA Kit is specifically designed for the precise detection of CCR1 levels in human serum, plasma, and cell culture supernatants. With superior sensitivity and specificity, this kit ensures accurate and consistent results, making it an ideal tool for a wide range of research applications.CCR1 is a key receptor involved in the chemotaxis of immune cells, playing a critical role in inflammatory responses and the regulation of immune cell trafficking. Dysregulation of CCR1 has been implicated in various inflammatory diseases, autoimmune disorders, and cancer, highlighting its importance as a potential therapeutic target and biomarker for disease progression.
The Human CCR1 ELISA Kit provides researchers with a reliable and efficient method for quantifying CCR1 levels in biological samples, ultimately aiding in the understanding of its physiological and pathological roles. With its ease of use and robust performance, this kit is a valuable asset for studies aiming to unravel the complexities of immune response and inflammation.
Product Name:
Human Chemokine C-C-Motif Receptor 1 (CCR1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
